In this paper, we describe a novel dispersion tailored properties of surface plasmon resonance (SPR) sensor assisted by Teflon and metamaterial layers. The dispersion property and the excitation methods of surface plasmon (SP) waves have been great significance in sensor design. Most of the time, SPR sensor designed for biosensing has been dual mode and its side lobes in dispersion graph can significantly reduce the sensitivity. We propose a design of as many as five-mode SP sensor assisted by Teflon and metamaterial layers. A new sensing theory for exploring highly dispersion tailored properties of multilayer sensor structure is design for efficient biorecognition. The proposed design scheme is able to completely remove the side lobes in the dispersion graph by properly controlling the Teflon layer, thin gold film, and metamaterial thickness for the case of multimode SPR sensor. To the best of our knowledge, dispersion tailored properties of multimode SP sensor assisted by Teflon and metamaterial layers together have not been explored and analyzed. The standard matrix method is applied to calculate the various performance parameters of the sensor. Experimental validation has been done with available results for a specific case. © 2018 IEEE.