Struvite or magnesium ammonium phosphate hexahydrate (MAPI1) is one type uriitary stone that arc which occur due to the urinary tract infection. These stones are mainly caused due to the bacteria which splits urea. These stone develop rapidly to form "Staghorn calculi" that causes urological disorder which is very painful. In this method, sodium metasilicate (SMS) act as a substrate for development of struvite crystal in vitro. The crystal is developed using single diffusion technique which consist of inner and outer reactant. After the growth of struvite crystals, they were dissolved in various solvents like DMSO, DMF and ethanol (at 50 ppm, 100 ppm, 200 ppm, 300 ppin). The dissolved compounds underwent the antimicrobial activity with Escherichia coli, Acetobacter spp and Pseudomonas spp. © 2015 Scientific Publishers. All rights reserved.