Rhizosphere region are the most nutrient rich niche among the soilenvironment that feeds the soil dwelling microorganisms. Totally eight isolateswere obtained from the rhizosphere of sugarcane plant that were grown inagricultural fields of Vellore district. The study was further preceded for in vitro screening of plant growth promoting traits viz., production of Indole acetic acid(IAA), Hydrogen cyanide, Siderophore, solubilization of phosphate, N fixationand 1-aminocyclopropane-1- carboxylic acid (ACC) deaminase activity of theisolates. Siderophore and phosphate solubilization were screened qualitatively onchrome azurol S and Pikovskaya agar. All the isolates exhibited positively forTri-calcium phosphate solubilisation. The results showed that all isolatesproduced IAA ranging from 9.14 ± 0.27 to 32.32±0.98 µmol ml-1 in the presenceof L-Tryptophan. Five isolates displayed siderophore production and none of theisolates turned positive for Hydrogen cyanide production. Only three isolateswere able to cleave 1-aminocyclopropane-1-carboxylate. The bacterial isolatesshowed difference in plant growth promotion traits while the isolate Vsc-14exhibited multiple plant growth promotion activity.