Electrophysiological signal acquisition is a process that involves congregation of physiological signals that are very helpful in detecting a variety of ailments like epilepsy, sleep disorders, cardiac arrhythmia, best disease, muscular and nervous-system disorders, dominant cystoids macular dystrophy, brain death and coma. Electrophysiological signals can also be used for tracking the human kinesiology and also in enhancing human machine interaction. Electrophysiological signal acquisition has a long history of use in healthcare and research, but there are certain practical factors that can affect the electrophysiological signal acquisition. This work discusses about the practical factors affecting electrophysiological signal acquisition and also proposes some of the possible engineering solutions to overcome these factors. © 2016, International Journal of Pharmacy and Technology. All rights reserved.