The marine micro organisms are the store house of a wide variety of biologically active products. Among the vast population of microorganisms which include algae, bacteria, actinomycetes etc., marine fungi are considered as the most useful microorganisms in biotechnology field. The marine environment conditions the micro flora to generate metabolites that have antimicrobial qualities as a self- defending mechanism. The objective of this paper was to isolate the Zinc resistant fungal strains from marine samples like wood , seashore sand , sea water and wooden boat and identify the isolated cultures morphologically till the genus level. The antimicrobial compounds were extracted using different organic solvents and their antibacterial activity was cheked on the selected pathogenic bacteria at different varying concentration. A confirmatory test was performed for each test organism in their selective media. The fungal cultures were found to belong to the genus Aspergillus, Mucor and Fusarium. The extracts of hexane and chloroform on mucor showed very good activity on E.coli while average activity on Pseudomonas aeruginosa and Klebsiella pneumonia and least activity in S. aureus cultures was found.