One out of every third person in the world is a smoker. Most of these people are in the reproductive phase of life. Smoking is found to be one of the leading causes of not only lung cancer, respiratory and cardiovascular disorders but also infertility in men. This is due to the presence of oxidants in cigarette smoke. These contribute to an increase in the concentration of Reactive Oxygen Species. The hike in oxidative stress causes lipid peroxidation of the sperm membrane, thus disrupting its structure. As a result, there is a decrease in sperm motility which reduces its ability to fertilize the egg. This study deals with assays (Lipid Peroxidation assay and NBT Staining) that measure or indicate the ROS concentration and antioxidant concentration (Ascorbate and Superoxide Dimutase assay). Sperm stability is analysed by calculating Zeta potential.