Ecofriendly synthesis of Palladium nanoparticles (PdNPs) were achieved using Phyllanthus emblica (P. emblica) seeds as reducing agent. Further the ecofriendly synthesized PdNPs were subjected for various analytical techniques like UV–Vis, FT-IR, XRD, Zeta potential, SEM and TEM. The results indicated that green synthesized PdNPs were spherical in shape with average particle size of 28 ± 2 nm with moderate stability. Further the synthesized PdNPs and extract were subjected for its antibacterial studies against various disease causing pathogens by agar well diffusion method. Seed extract resulted in 8.9 ± 1.46 mm against B. subtilis and PdNPs showed 9.6 ± 1.10 mm against S. aureus and synthesized PdNPs and extract were tested for hemolytic which resulted in 20% and 10% respectively. Toxicity studies were done against Artemia salina (A. salina). The LC50 value of green synthesized P. emblica capped PdNPs and the P. emblica seed extract were found to be less toxic for A. salina with a value of 1.00 μg/mL and 1.25 μg/mL. In addition samples were checked for in vitro cytotoxicity assays on HeLa cell lines. © 2016 Elsevier B.V.