The purpose of the current study is to monitor the phytochemical constituents in Tinospora cordifolia stem extract. Phytochemical screening of the sequential extracts were used for analysis which showed the presence of bioactive compounds like alkaloids, flavonoids, phenols, tannins, saponins, terpenoids and carbohydrates. Ultra violet visible spectroscopy was used to recognize quantitative determination of different analytes by using wavelength and absorbance values. FT-IR analysis was used to identify the functional groups of the compounds. Four functional groups were identified such as aromatics (C-H), secondary amines and amides (N-H), α, β- unsaturated aldehydes and ketones (C=O) and alkyl halides (C-H). GC-MS analysis for methanolic stem extract was done. The compound 3, 7, 11, 15-Tetramethyl-2-Hexadecen-1-ol reported to possess analgesic, anti-inflammatory and antipyretic activities, 9- Eicosene, (e) has anticancer activity, Hexamethyl-Cyclotrisiloxane, has antibacterial activity. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.