Xylanases are a group of hemicellulolytic extracellular enzyme required for the hydrolysis of β-1, 4-xylans present in lignocellulosic materials. In this study, xylanase producing bacteria Bacillus weihenstephanensis strain ANR1 was isolated from the soil. The production of xylanase enzyme was carried out by submerged fermentation utilizing various lignocellulosic substrates such as corn cobs, orange peel, rice bran, water melon rind, grape pomace, pomegranate peel, musk melon rind and pineapple peel at 1% (w/v). Among the substrate used watermelon rind produced the maximum of 426 U/ml of xylanase enzyme. The cellulose free xylanase enzyme was active and stable at the pH of 7 and the enzyme retained its activity at 37ºC. Based on the results obtained that this superior property of xylanase enzyme produced from Bacillus weihenstephanensis strain ANR1 makes it ideal for the application in food industry. © 2015, Sphinx Knowledge House. All rights reserved.