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Screening and optimization of polyhydroxyalkanoate (PHA) produced by Bacillus drentensis VIT-SSR3 isolated from industrial effluent contaminated soil
S. Evangeline,
Published in World Research Association
2018
Volume: 13
   
Issue: 8
Pages: 79 - 84
Abstract
Petroplastics have caused a negative impact on the environment and hence this has led to the search for bioplastics with superior material properties. Unlike petroplastics, Polyhydroxyalknoates (PHAs) biopolymers were synthesized by a vast genus of microorganisms intracellularly as carbon and energy reserve materials. The aim of the study was to isolate novel bacteria capable of accumulating polyhydroxyalkanoate (PHA) from an industrial effluent contaminated soil environment. All the isolates obtained were screened for PHA production by Sudan Black B staining and Nile Blue A staining, of which one isolate VIT-SSR3 was found to have potential PHA accumulation capacity. The biochemical and phenotypic analysis identified the isolate as Bacillus drentensis VIT-SSR3. The maximum PHA content of (0.186±0.003) g/L was obtained when glucose was used as the carbon source and the maximum PHA yield of (33.08±0.30) % was obtained when grown on ammonium sulphate as the nitrogen source. The optimum pH and temperature for maximum PHA production were determined to be 7 and 35°C respectively. The produced biopolymer was analyzed using Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). © 2018 World Research Association. All rights reserved.
About the journal
JournalResearch Journal of Biotechnology
PublisherWorld Research Association
ISSN09736263