Nanoparticle based agents often applied as coatings on biomaterials have shown promise in providing improved sterility against variety of microbes. In the present study, silk fibers were coated with poly (2-hydroxyethyl methacrylate) (pHEMA), impregnated with silver nanoparticles and coupled with ciprofloxacin. The prepared composite material was characterized for its physico-chemical properties and in vitro cell proliferation tests were performed using 3T3 fibroblast cell lines. The infrared spectroscopy results confirmed the protein nature of the biomaterial. The surface morphology results of SEM images demonstrate silk fibroin coated with pHEMA along with impregnated silver nanoparticles. The biomaterial exhibits improved thermal stability, enhanced antimicrobial activity and better cell proliferation along with adhesion property, thereby making it ideal candidate as biomaterial for wound dressings. The efficacy of the biomaterial for wound dressing and as tendon reconstruction material could be further evaluated using small animals.