Polythiophene nanofibers have been prepared by in situ chemical oxidative polymerization method. X-ray diffraction method (XRD) and Fourier transform infrared analysis (FTIR) were used to confirm the formation of polythiophene. The synthesised polythiophene nanofibers were also characterized by energy dispersive analysis of X-rays (EDAX), transmission electron microscopy (TEM) and atomic force nicroscopy (AFM). Zeta potential measurement was employed to determine the surface charge of the polymer.