This work presents a novel approach for functionalization of silver nanoparticles and cross-linking them with collagen to form FSCSC scaffolds suitable for clinical applications.

Sekar  S K

Department of Structural and Geotechical Engineering

School of Civil Engineering

Vellore Campus

Amitava Mukherjee

Centre for Nanobiotechnology

Chandra Sekaran N

Mandal A

Sastry T.P.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

&nbsp;

&nbsp;

VIT University

Journal of Materials Chemistry B

Clean Technologies and Environmental Policy

Effect of reinforced Al2O3 nanoparticles on collagen nanobiocomposite from chrome-containing leather waste for biomedical applications

Vibrational spectroscopies as analytical tools to investigate the interaction of sago starch-capped silver nanoparticles with collagen scaffolds for biomedical applications.

RSC Advances

Vibrational spectroscopic investigation on interaction of sago starch capped silver nanoparticles with collagen: a comparative physicochemical study using FT-IR and FT-Raman techniques

Silver nanoparticles capped with sago starch that were used for the fabrication of biocompatible collagen-based scaffolds may find tissue regeneration applications.

Phys. Chem. Chem. Phys.

Fabrication of collagen scaffolds impregnated with sago starch capped silver nanoparticles suitable for biomedical applications and their physicochemical studies

Silver and magnetic (Fe3O4) nanoparticles have attracted wide attention as novel antimicrobial agents due to their unique chemical and physical properties. In order to study the comparative effects on antibacterial and animal cytotoxicity, Staphylococcus aureus and NIH 3T3 fibroblasts were used, respectively. Both nanoparticles were synthesized via a novel matrix-mediated method using poly(ethylene) glycol. Formation of silver nanoparticles was confirmed by fluorescence and ultraviolet-visible spectroscopic techniques. The poly(ethylene) glycol-coated silver and Fe3O4 nanoparticles were characterized by scanning electron microscope, transmission electron microscope, zeta potential, particle size analysis, Fourier-transform infrared, X-ray diffraction, and X-ray photoelectron spectroscopy. The antimicrobial results indicate that both poly(ethylene) glycol-coated silver and Fe3O4 nanoparticles inhibited S. aureus growth at the concentrations of 5 and 10 µg/mL at all time points without showing any significant cytotoxicity on NIH 3T3 fibroblasts. The particle size of both the poly(ethylene) glycol-coated silver and Fe3O4 nanoparticles dominated in the range 10-15 nm, obtained by particle size analyzer. The poly(ethylene) glycol coating on the particles showed less aggregation of nanoparticles, as observed by scanning electron microscope and transmission electron microscope. The overall obtained results indicated that these two nanoparticles were stable and could be used to develop a magnetized antimicrobial scaffolds for biomedical applications.

Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine

Poly(ethylene) glycol–capped silver and magnetic nanoparticles: Synthesis, characterization, and comparison of bactericidal and cytotoxic effects

Background In this study, an attempt has been made with the advent of technology to prepare a multifunctional nanobiocomposite (NBC) for targeted drug delivery in cancer therapy. Methods Collagen (C) was fabricated as nanofibers with multifunctional moieties viz. CFeAb*D by incorporating iron oxide nanoparticles (Fe), coupling with fluorescein isothiocyanate (FITC) labeled antibody (Ab*) and loading an anticancer gemcitabine drug (D). This NBC was characterized by conventional methods and evaluated for its biological activities. Results The UV-vis and FTIR spectroscopic studies revealed the fluorescein to protein ratio and revealed the presence of iron oxide nanoparticles and their interaction with the collagen molecules, respectively. While SDS-PAGE showed the proteinaceous nature of collagen, VSM and TEM studies revealed magnetic saturation as 54.97 emu/g and a magnetic nanoparticle with a diameter in the range of 10-30 nm and the dimension of nanofiber ranging from 97 to 270 nm. A MRI scan has shown a super paramagnetic effect, which reveals that the prepared NBC can be used as a MRI contrast agent. The MTT assay has shown biocompatibility and an apoptotic effect while phase contrast microscopy exhibited receptor mediated uptake of endocytosis. Conclusion The novelty in the prepared NBC lies in the collagen nanofibers, which have a higher penetrating property without causing much cell damage, biocompatibility and multifunctional properties and is able to carry multifunctional agents. General significance The study has demonstrated the possible use of CFeAb*D as a multifunctional NBC for biomedical applications. Â© 2013 Elsevier B.V.

Biochimica et Biophysica Acta (BBA) - General Subjects

Collagen based magnetic nanobiocomposite as MRI contrast agent and for targeted delivery in cancer therapy

Drug Discovery Today

Silver nanoparticles: synthesis, properties, and therapeutic applications

Synthesis, characterization and evaluation of collagen scaffolds crosslinked with aminosilane functionalized silver nanoparticles: in vitro and in vivo studies

Journal	Data powered by TypesetJournal of Materials Chemistry B
Publisher	Data powered by TypesetRoyal Society of Chemistry (RSC)
ISSN	2050-750X
Open Access	0