Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

BACKGROUND: Perceived organizational support (POS) measures the employee perception of support from the organization and helps to understand the employer-employee relationship better. The aim of the study is to understand the healthcare professionals' perception of support from public health department and to examine thehealthcare professionals' difference in perception of support across various demographics variables. METHODS: A survey is conducted among healthcare professionals including medical officers and staff nurses working in primary health centres (PHC) in the state of Tamilnadu. In the study, public health department refers to the Directorate of Public Health and Preventive Medicine. Mann Whitney U test and Kruskal Wallis H test are used to assess the difference in POS across demographic variables. RESULTS: The results of the study indicated that the healthcare professionals perceive a moderate levelof support from public health department. The healthcare professionals' POS is found to differ across their age, role and total work experience but not across gender and work experience in the current PHC. Further, the POS of healthcare professionals is found to differ with respect to PHC location and does not differ with respect to PHC type. CONCLUSION: The results emphasize the need to enhance POS of healthcare professionals. The health policy makers and mangers have to consider the difference in POS of healthcare professionals and make amendments in the human resource policies related to selection and training.

Management

VIT Business School

Chennai Campus

Department of English

School of Social Sciences & Languages

Vellore Campus

VIT Unitversity

Fulltext

Indian Journal of Public Health

Healthcare professionals′ perspective of support from public health department : a0 study in the primary health centers of Tamil Nadu

Objective: The aim of the present study was to assess the anti-Aspergillus activity of culture filtrate of Streptomyces sp. VITSTK7 and biologically synthesized Ag2O/Ag nanoparticles using the culture filtrate of VITSTK7. Material and methods: Silver nanoparticles were synthesized by biological reduction of silver nitrate using culture filtrate of Streptomyces sp. VITSTK7. The synthesized nanopaticles were characterized for surface plasma resonance peak, X-ray diffraction patterns, shape and size distribution. Inhibition of mycelial growth was used as an index of anti- Aspergillus activity of synthesized nanoparticles. Results: The synthesized nanoparticles were spherical shaped and monodespersive in nature and showed a single surface plasma resonance peak at 420. nm. X-ray diffraction patterns displayed typical peaks of crystalline silver oxide and size distribution histograms indicated production of 20-60-nm-size nanoparticles with average size of 35.2. nm. The synthesized nanoparticles showed anti- Aspergillus activity against Aspergillus niger, Aspergillus flavus and Aspergillus fumigatus with antifungal index in the range of 62-75%. Conclusion: Thus the bioconversion of Ag2O/Ag nanoparticles by Streptomyces sp. VITSTK7 could be employed to control Aspergillus pathogenesis. The results of this study suggest that the green synthesis of Ag2O/Ag nanoparticles using marine Streptomyces sp. VITSTK7 yielded 27.9nm sized particles with potential to act against pathogenic Aspergillus species. © 2013 Elsevier Masson SAS.

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Computer Science

School of Computer Science and Engineering

Journal de Mycologie Médicale

Antifungal activity of Streptomyces sp . VITSTK7 and its synthesized Ag 2 O/Ag nanoparticles against medically important Aspergillus pathogens

Antifungal drugs are inhibitors either of fungal cell wall biosynthesis or essential reaction steps of fungal metabolic pathways. In silico studies have proved to be very effective on screening small molecules to be used as drugs and identifying essential reactions and pathways as targets. The aim of the present study was to predict the interactions of compounds present in the ethyl acetate extract of Streptomyces sp. VITSTK7 against selected fungal drug target enzymes. The ethyl acetate extract of the isolate showed significant anti-Aspergillus activity against the selected Aspergillus pathogens. Presence of the three compounds (C22H37NO7, C17H24N4O6 and C24H28N2O5) in the extract was identified by GC-MS spectra and matched with reference compounds available in the MS spectra library, NIST (National Institute for Standards and Technology). These compounds were analysed for the interaction with five selected fungal target proteins 1AFR, 1EA1, 1LKP, 1ZHX and 3PD73i3E. Docking was done using Patch dock beta 1.3 version and analysed by pymol 1.3 version. The tested compounds C22H37NO7, C17H24N4O6 and C24H28N2O5 showed least binding energy of -254.64 kcal/mol, -248.71 kcal/mol and -338.57 kcal/mol respectively with 1ZHX. The result of this study revealed that all the three compounds from the strain had higher interaction with 1ZHX protein than with the other proteins. It shows that this strain could be the promising source for the antifungal drug. © 2013 International Association of Scientists in the Interdisciplinary Areas and Springer-Verlag Berlin Heidelberg.

Interdisciplinary Sciences: Computational Life Sciences

Interaction of Streptomyces sp. VITSTK7 compounds with selected antifungal drug target enzymes by in silico molecular docking studies

The conventional method of synthesizing gold nanoparticles (Au NPs) by chemical methods tends to cause an environmental concern; therefore there is an increasing tendency to use safe and nontoxic methods. The aim of the present study was to evaluate the anti-dermatophytic potential of biologically synthesized Au NPs using the cell free supernatant of Streptomyces sp. VITDDK3. The synthesized Au NPs were characterized using UV-visible spectra at 550 nm. XRD pattern analysis revealed peaks at 38.10° (111), 44.28° (200), 64.42° (220) and 77.37° (311) corresponding to the diffraction facets of Au. SEM analysis showed that the Au NPs were cubical in shape with an average size of 90 nm. The synthesized Au NPs were tested by the well diffusion method for antifungal activity (zones of inhibition) against Microphyton gypseum (10±0.47 mm) and Trichophyton rubrum (13±1.03 mm). © 2012 Published by Elsevier B.V. All rights reserved.

Department of Analytics

Materials Letters

Actinobacteria mediated synthesis of gold nanoparticles using Streptomyces sp. VITDDK3 and its antifungal activity

Ticks and mosquitoes are ectoparasitic arthropods that can transmit a variety of diseases to humans and animals during blood feeding and causing serious infectious disorders. The purpose of the present study was to assess the acaricidal and insecticidal property of ethyl acetate extract and its compounds isolated from marine actinobacteria, Streptomyces VITSTK7 sp. against the larvae of cattle ticks, Haemaphysalis bispinosa and Rhipicephalus (Boophilus) microplus (Acari: Ixodidae); fourth-instar larvae of malaria vector, Anopheles subpictus; and filarial vector, Culex quinquefasciatus (Diptera: Culicidae). The ethyl acetate extract was loaded on silica gel column and separated with chloroform, methanol, and acetone as the solvents system. The separation of fractions was visualized by the thin layer chromatography (TLC) plate, further confirmed by high-performance liquid chromatography, and followed by gas liquid chromatography. Three major fractions were analyzed in mass spectroscopy (MS) and matched with existing compounds in the data base. Based on the fragment pattern, it led to the major compounds which were predicted as cyclopentanepropanoic acid, 3,5-bis(acetyloxy)-2-[3-(methoxyimino)octyl], methyl ester (13.3 %) 1; 5-azidomethyl-3-(2-ethoxy carbonyl-ethyl)-4- ethoxycarbonylmethyl-1H-pyrrole-2-carboxylic acid, ethyl ester (18.2 %) 2; and akuammilan-16-carboxylic acid, 17-(acetyloxy)-10-methoxy, methyl ester (16R) (53.3 %) 3. The maximum efficacy was observed in compounds 1, 2, and 3, and the ethyl acetate extract of Streptomyces VITSTK7 sp. against the larvae of H. bispinosa (LC50 = 1,573.36, 1,333.09, 1,073.29, and 409.71 ppm; r 2 = 0.0.990, 0.934, 0.935, and 0.908), R. microplus (LC50 = 1,877.86, 815.83, 1,631.14, and 441.54 ppm; r 2 = 0.981, 0.926, 0.0970, and 0.915), A. subpictus (LC50 = 273.89, 687.69, 464.75, and 223.83 ppm; r 2 = 0.758, 0.924, 0.841, and 0.902), and C. quinquefasciatus (LC50 = 430.06, 881.59, 777.0, and 195.70 ppm; r 2 = 0.839, 0.859, 0.870, and 0.882), respectively. Results of the present study provide evidence that the maximum parasitic activity of ethyl acetate extract and a synergistic effect of combinations of different compounds have been suggested. The control (distilled water) showed nil mortality in the concurrent assay. In the present study, a novel, targeted, simple, and eco-friendly approach has been suggested to control blood-feeding parasites. © 2012 Springer-Verlag Berlin Heidelberg.

Parasitology Research

Eco-friendly approach using marine actinobacteria and its compounds to control ticks and mosquitoes

The title compound, C9H8N4, crystallizes with three independent mol-ecules (A, B and C) per asymmetric unit. The independent mol-ecules differ slightly in their conformations, the dihedral angles between the triazole and phenyl rings in mol-ecules A, B and C being 4.8 (2), 9.7 (2) and 7.2 (2)°, respectively. In the crystal, the independent mol-ecules are linked into a trimer by C - H⋯N hydrogen bonds.

Department of Chemistry

School of Advanced Sciences

Acta Crystallographica Section E Structure Reports Online

(E)-N-Benzylidene-4H-1,2,4-triazol-4-amine

In the title compound, C(18)H(21)NO(3), which belongs to the family of calcium channel blockers, the dihydropyridine ring assumes a flattened boat conformation. The two carbonyl units adopt a synperiplanar conformation with respect to the double bonds in the dihydro-pyridine ring. The methoxy-phenyl ring is almost perpendicular to the prydine ring [dihedral angle = 89.01 (7)°]. In the crystal, the mol-ecules are connected by inter-molecular N-H⋯O hydrogen bonds.

1,1′-[4-(4-Methoxyphenyl)-2,6-dimethyl-1,4-dihydropyridine-3,5-diyl]diethanone

In the title compound, C15H18N2O 5·H2O, the pyrimidine ring adopts a flattened-boat conformation. The eth-oxy group attached to the benzene ring is in an extended conformation. The oxopyrimidine mol-ecules are linked into centrosymmetric R 2 2(20) dimers by O - H⋯O hydrogen bonds. The dimers are linked by N - H⋯O hydrogen bonds, forming a two-dimensional network parallel to the bc plane. Adjacent networks are cross-linked via N - H⋯O and O - H⋯O hydrogen bonds involving the water mol-ecules. © 2009 Thenmozhi et al.