The objective of this work is eco-friendly synthesis of Carbon/Copper oxide nano composites (C/CuO nano composites) from the aqueous extract of Aristolochia bracteolata (ABLPAE) via response surface methodology (RSM). Here we optimized various parameters to find out the absorption maximum value using RSM technique. Then the synthesized C/CuO nano composites were processed using different analytical tools. The crystanality of C/CuO was investigated using XRD analysis which exhibited crystalline size of 43 ± 2 nm. Additionally, synthesized C/CuO nano composites were used to study larvicidal activity using MANOVA; LSD-DMRT test. We found that results showed LC50 value at 26.42 ppm and 86.13 ppm for LC90. Ovicidal activity by anti-feedant method resulted in a dose dependent manner with a high mortality rate at higher concentrations. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.