Electrochemical anodization is a unique surface modification technique for modifying the titanium surface. Electrochemical alteration of titanium surface increases the material efficiency in biomedical applications. The present research work analyses the fabrication of TiO2 nanotubes by increasing the water content and the various results and characterization enhance the cell viability. The influence of water content in electrolytes improves cell viability and at the same time, it is nontoxic. The surface morphologies were studied with HR-SEM, phase transformation was characterized using X-ray diffraction and cell viability was investigated with MTT assay by NIH-3T3 fibroblast cells incubation time for 48 hours (standard time incubation). © 2021 Trans Tech Publications Ltd, Switzerland.